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Peer-Reviewed Open Access Publication · ISSN: 2321-8681 · CC-BY 4.0 International

Open Access ISSN: 2321-8681 Volume-6, Issue-1 2019

Fluorescence spectroscopy based folding-unfolding of Protein [Bovine Serum Albumin (BSA)]: an UG/PG experiment in Biotechnology

Authors: Arun Dev Sharma, Priya Nischal, Narvir Singh, Jagpreet Singh Nanda India
Correspondence: arundevsharma47@gmail.com
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Abstract

Eukaryotic and prokaryotic cells contain number of endoflores like proteins which undergoes folding-unfolding process and play very crucial biological role inside the cells. In this study an under-graduate (UG) and post-graduate (PG) experiment was set up to study folding-unfolding of protein BSA. It was observed that fluorescence of the protein in solutions varied as per the concentration of urea and guanidine hydrochloride. It was postulated that fluorescence spectroscopy based approach can be used to document protein folding process to the students of UG and PG classes.

https://doi.org/10.6084/m9.figshare.8988713.v2

Journal Research and Reviews in Biotechnology and Biosciences
Peer Review Double-Blind Peer Reviewed
Publication Year 2019
Volume Volume-6, Issue-1
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Arun Dev Sharma, Priya Nischal, Narvir Singh, Jagpreet Singh Nanda (2019 ). Fluorescence spectroscopy based folding-unfolding of Protein [Bovine Serum Albumin (BSA)]: an UG/PG experiment in Biotechnology. Research and Reviews in Biotechnology and Biosciences, Volume-6, Issue-1.
Arun Dev Sharma, Priya Nischal, Narvir Singh, Jagpreet Singh Nanda, 2019 . Fluorescence spectroscopy based folding-unfolding of Protein [Bovine Serum Albumin (BSA)]: an UG/PG experiment in Biotechnology. Research and Reviews in Biotechnology and Biosciences, Volume-6, Issue-1.
Arun Dev Sharma, Priya Nischal, Narvir Singh, Jagpreet Singh Nanda. "Fluorescence spectroscopy based folding-unfolding of Protein [Bovine Serum Albumin (BSA)]: an UG/PG experiment in Biotechnology." Research and Reviews in Biotechnology and Biosciences Volume-6, Issue-1 (2019 ).
@article{rrbb_91_ArunDevS, title = {{Fluorescence spectroscopy based folding-unfolding of Protein [Bovine Serum Albumin (BSA)]: an UG/PG experiment in Biotechnology}}, author = {Arun Dev Sharma, Priya Nischal, Narvir Singh, Jagpreet Singh Nanda}, journal = {Research and Reviews in Biotechnology and Biosciences}, issn = {2321-8681}, year = {2019 }, volume = {Volume-6, Issue-1} }
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