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Peer-Reviewed Open Access Publication · ISSN: 2321-8681 · CC-BY 4.0 International

Open Access ISSN: 2321-8681 Volume-1, Issue-2 2014

Production of Alkaline Protease by Bacillus Circulans Using Submerged Fermentation, Their Purification and Characterization

Authors: Inderjeet Kaur, Ramandeep Kaur India
Correspondence: kaurinder@lkc.ac.in
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Abstract

In the present study, an extracellular alkaline protease of Bacillus circulans was cultivated on modified Reese medium at a pH of 9.5, temperature of 28oC in 168 hours of incubation. This strain was purified 16.76 folds with specific activity of 1101.0 U/mg in comparison to crude extract using ammonium sulphate precipitation (30-60%), dialysis and DEAE-Cellulose anion exchange chromatography. The molecular weight of the purified enzyme was found to be 30 kDa on Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The characterization of purified enzyme revealed 9.5 and 70°C as an optimum pH and temperature, respectively. The enzyme was activated by Ba2+, Ca2+, Mn2+, Co2+ and Zn2+ and inhibited by Hg2+ and EDTA. The results thus suggested that the alkaline protease of B. circulans is a metalloprotease with serine at its active centre. The specific activity of this alkaline protease from Bacillus circulans is greater than those of other reported Bacillus sp; therefore, it was concluded that it may be suitable candidate for commercial applications.

Journal Research and Reviews in Biotechnology and Biosciences
Peer Review Double-Blind Peer Reviewed
Publication Year 2014
Volume Volume-1, Issue-2
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Inderjeet Kaur, Ramandeep Kaur (2014). Production of Alkaline Protease by Bacillus Circulans Using Submerged Fermentation, Their Purification and Characterization. Research and Reviews in Biotechnology and Biosciences, Volume-1, Issue-2.
Inderjeet Kaur, Ramandeep Kaur, 2014. Production of Alkaline Protease by Bacillus Circulans Using Submerged Fermentation, Their Purification and Characterization. Research and Reviews in Biotechnology and Biosciences, Volume-1, Issue-2.
Inderjeet Kaur, Ramandeep Kaur. "Production of Alkaline Protease by Bacillus Circulans Using Submerged Fermentation, Their Purification and Characterization." Research and Reviews in Biotechnology and Biosciences Volume-1, Issue-2 (2014).
@article{rrbb_89_Inderjeet, title = {{Production of Alkaline Protease by Bacillus Circulans Using Submerged Fermentation, Their Purification and Characterization}}, author = {Inderjeet Kaur, Ramandeep Kaur}, journal = {Research and Reviews in Biotechnology and Biosciences}, issn = {2321-8681}, year = {2014}, volume = {Volume-1, Issue-2} }
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