Paper Details

Name: Parul Gulati, Arun Dev Sharma
Email: arundevsharma47@gmail.com
Country: India

Year: 2014
Volume: Volume-1, Issue-1

The effect of phosphorus stress on changes in acid phosphatase (APase) activities was studied in wheat seeds and shoots. Six-day old wheat seedlings were exposed to low phosphorus (LP) and high phosphorus (HP) stress by providing two different concentrations of 10μM and 1000μM KH2PO4 .The tissues (seeds and shoots) were separated . Treated and untreated extracts of seeds and shoots were compared. The Acid phosphatase activity was measured by using p-nitrophenyl phosphate (pNPP) as substrate, results revealed that APase activity was strongly induced under phosphorus stress. NATIVE PAGE APase activity also revealed a similar type of induction under phosphorus stress. SDS PAGE Analysis revealed that many polypeptides in treated extracts disappeared as compared to untreated extracts Based on these results; role of acid phosphatase (APase) was studied.