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Peer-Reviewed Open Access Publication · ISSN: 2321-8681 · CC-BY 4.0 International

Open Access ISSN: 2321-8681 Volume: 13, Issue: 2 (July-December) 2026

Socks Flora of Young Male Adults -Enterobacteriaceae and Pseudomonas Spp. Predominates

Authors: Bharathi Prakash, Sumangala C H, Deepa, Manjunatha B.S, Hrishikesha, Udaya Kumar, Sushmitha, Aishwarya J Devadiga, Rashmi India

Abstract

Socks provide comfort and protection to the feet but can also serve as a suitable environment for microbial growth due to the accumulation of sweat, moisture, warmth and nutrients. The present study was conducted to isolate and identify the bacterial microflora associated with worn socks. A total of 10 socks samples were collected from healthy male volunteers aged 15–30 years using sterile swabs. Swab samples were inoculated onto Nutrient Agar and MacConkey Agar and incubated aerobically at 37°C for 24 hours. The isolates were characterized based on colony morphology, Gram staining and biochemical tests. The study revealed a diverse microbial population associated with the sock samples. Gram-negative bacilli were predominant, while Staphylococci and Actinomycetes were also detected in some samples. The bacterial isolates included Klebsiella spp., Escherichia coli, Pseudomonas spp., Citrobacter spp., Enterobacter spp. along with Bacillus species. The findings demonstrate that socks can harbour diverse microorganisms, including potentially opportunistic pathogens. The warm and humid conditions inside socks and footwear may favour microbial multiplication and contribute to unpleasant foot odour. Proper washing, thorough drying and regular changing of socks are therefore important for maintaining foot hygiene. The study highlights the microbiological significance of socks, its cleanliness and the potential need for antimicrobial approaches to reduce socks microflora thereby improving foot hygiene.

Keywords
Socks microflora; Gram staining; Escherichia coli; Klebsiella spp.; Pseudomonas spp. Antimicrobial approaches

Journal Research and Reviews in Biotechnology and Biosciences
Peer Review Double-Blind Peer Reviewed
Publication Year 2026
Volume Volume: 13, Issue: 2 (July-December)
Page Range 28-36
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Bharathi Prakash, Sumangala C H, Deepa, Manjunatha B.S, Hrishikesha, Udaya Kumar, Sushmitha, Aishwarya J Devadiga, Rashmi (2026). Socks Flora of Young Male Adults -Enterobacteriaceae and Pseudomonas Spp. Predominates. Research and Reviews in Biotechnology and Biosciences, Volume: 13, Issue: 2 (July-December) , 28-36. https://doi.org/10.5281/zenodo.22096038
Bharathi Prakash, Sumangala C H, Deepa, Manjunatha B.S, Hrishikesha, Udaya Kumar, Sushmitha, Aishwarya J Devadiga, Rashmi, 2026. Socks Flora of Young Male Adults -Enterobacteriaceae and Pseudomonas Spp. Predominates. Research and Reviews in Biotechnology and Biosciences, Volume: 13, Issue: 2 (July-December) , pp. 28-36.
Bharathi Prakash, Sumangala C H, Deepa, Manjunatha B.S, Hrishikesha, Udaya Kumar, Sushmitha, Aishwarya J Devadiga, Rashmi. "Socks Flora of Young Male Adults -Enterobacteriaceae and Pseudomonas Spp. Predominates." Research and Reviews in Biotechnology and Biosciences Volume: 13, Issue: 2 (July-December) (2026): 28-36.
@article{rrbb_324_BharathiP, title = {{Socks Flora of Young Male Adults -Enterobacteriaceae and Pseudomonas Spp. Predominates}}, author = {Bharathi Prakash, Sumangala C H, Deepa, Manjunatha B.S, Hrishikesha, Udaya Kumar, Sushmitha, Aishwarya J Devadiga, Rashmi}, journal = {Research and Reviews in Biotechnology and Biosciences}, issn = {2321-8681}, year = {2026}, volume = {Volume: 13, Issue: 2 (July-December) }, pages = {28-36}, doi = {https://doi.org/10.5281/zenodo.22096038} }
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