Paper Details

Name: Ginjinder Kaur, Aayushi Walia & Pupinder Kaur
Email: aayushi_rs25@pbi.ac.in
Country: India

Year: 2026
Volume: Volume: 13, Issue: 1, (January-June)
Page Number: 70-80
DOI: https://doi.org/10.5281/zenodo.19843269

ABSTRACT
Background: Coronary heart disease is one of the leading cause of sudden cardiac death. Genetics play 40-60% role in the pathogenesis of disease, making it an important biomarker for determining risk in the population of Malwa region of Punjab. The gene F2 or Coagulation Factor II is responsible for
formation of an activated serine protease thrombin. This activated thrombin enzyme converts fibrinogen to fibrin during the formation of blood clot, by initiating aggregation of platelets in the process of thrombosis and hemostasis.

Methods: The case-control study was done to check the polymorphism in SNP. A total of 400 blood samples were taken and DNA was extracted using salting out method. PCRRFLP was done to detect mutation in SNP rs1799963 using HindIII restriction enzyme and specific set of primers. Statistical analysis was done to check the significance of results.

Results: The results of genotyping showed that both the case and control groups had greater percentage of wild allele type whereas only CHD patients had a mutant allele. The differences in allele frequencies were statistically significant for cases and controls (p = 0.0001, OR = 0.025; 95% CI = 0.001-0.43). For wild type G allele, the cases had a frequency of 95.5% (382/400) and for controls, it was 100% (400/400) whereas, for mutant allele A, the cases showed a frequency of 4.5% (18/400) and no control subject was detected with a mutant allele.

Conclusion: The results of present study illustrated that SNP rs1799063 was considered as a significant risk factor for developing CHD in females as compared to males. The study results showed that advancing age, gender, smoking, alcohol intake, blood pressure, height and weight were significant risk factors associated with coronary heart disease.

Key Words: Coronary Heart Disease, Prothrombin, Coagulation Factor II, CHD, Myocardial Infarction