Paper Details

Name: Arshdeep Kaur
Email: arrashdeep1996@gmail.com
Country: India

Year: 2025
Volume: Volume: 12, Issue: 2, (July-December)
Page Number: 75-87
DOI: https://doi.org/10.5281/zenodo.17130576

ABSTRACT:
In vitro fertilization (IVF) success relies heavily on accurate
embryo selection to enhance implantation and live birth rates.
Traditional methods, including morphological grading and
preimplantation genetic testing for aneuploidy (PGT-A), have
shown limitations in consistently predicting embryo viability.
Mitochondrial DNA (mtDNA) content has emerged as a
promising biomarker reflecting the bioenergetic and
developmental potential of embryos. This systematic review
synthesizes current evidence from human and animal studies
evaluating the association between mtDNA copy number and
implantation outcomes. Overall, higher mtDNA levels in
euploid embryos were often associated with reduced
implantation rates, independent of embryo morphology and
maternal age. Contradictory findings highlight the impact of
methodological variability, normalization strategies, and
biological factors. While mtDNA profiling shows potential as a
non-invasive adjunct for embryo selection, standardization of
measurement techniques and further large-scale studies are
needed to optimize its clinical application in assisted
reproductive technologies.
Keywords: Assisted reproductive technology (ART),
Biomarker, Embryo selection, Euploid embryos, In vitro
fertilization (IVF), Implantation, Mitochondrial DNA
(mtDNA).