Paper Details

Name: Harsha Sharma, Lakshay Vashishtha, Manik Sharma
Email: vashishthaharsha5@gmail.com
Country: India

Year: 2025
Volume: Volume-12, Issue-1 (January-June)
Page Number: 86-103
DOI: https://doi.org/10.5281/zenodo.16358282

Due to its potential therapeutic properties in preventing and
treating various disorders, the Alstonia scholaris leaf extract has
been designated as an anticancer and central nervous system (CNS)
modulating agent. The leaves of this Plant have a variety of
activities related to their constituents, which was observed by
Extraction using various types of Solvent according to their
polarities, various literature and research articles reported the
presence of most of the constituent, and here 2,3-secernate was
selected as an active constituent in treatment of Cancer of various
cell line (A549, HeLa, HepG2, HL60, and KB), respectively. In
this activity, it was seen against Breast cell lines MCF-7 and T47D
in Breast Carcinoma, and pyridine was used as a CNS-modulating
agent in Swiss albino mice with a body weight of between 20 and
25 grams. The activity was measured by locomotor and rotarod
activity. MTT-Assay was used to conduct in vitro cytotoxicity
research on a 69-year-old female organism of the species Homo
sapiens. Inactive mitochondria prevent even recently dead cells
from efficiently cleaving MTT. Live cells create a dark blue
formazan from MTT [3-(4,5-dimethyl thiazole-2-yl)-2,5-diphenyl
tetrasodium bromide], a light-yellow substrate. Thus,
calorimetrically assessing MTT cleavage may quantify the number
of living cells. The percentage of growth inhibition also increased
with increasing concentrations (in g/ml) of the ethanolic extract of
Alstonia scholaris, which included the separated component C.
This shows that the extract is very effective against cancer. Swiss
albino mice weighing 20–25 grams were exposed to a chemical
called pyridine, which altered their behavior. At doses of 50 and
100 milligrams, both the aqueous and ethanolic extracts showed
CNS depressive effects, as measured by a decrease in locomotor
activity counts and a delay in rotarod activity decrease.
Keywords: A549 cells, HeLa cells, HepG2 cells, MTT, Alstonia
Scholaris