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A Biannual Peer Reviewed, Refereed Research Journal (Estd. Year: 2014)

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Archives Volume-6, Issue-2

TABLE OF CONTENTS

Paper Title:
Efficacy analysis of various target tissues for genetic transformation to overcome recalcitrance in perennial tree crops
Author Name:
Rekha K1, Jayasree P K1,Jayashree1,R and Thomas K U1
Author Email:
thomasku79@gmail.com
Country:
India
DOI:
10.5281/zenodo.3459478
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Efficacy analysis of various target tissues for genetic transformation to overcome recalcitrance in perennial tree crops
Author: Rekha K1, Jayasree P K1,Jayashree1,R and Thomas K U1

Recalcitrance and low regeneration rate of perennial tree crops are the major hurdles faced by biotechnologist working on the development of transgenic tree varieties around the world. The nature of target tissue is a major deciding factor which determines the success in various stages of transgenic development like transformation, plant regeneration and hardening. Heveabrasiliensis, the major source of natural rubber is a perennial tree crop and the aforementioned problems have slow downed the efforts to developtransgenic Heveavarities with better agronomic traits. To overcome these issues in Hevea, an attempt was made in this study to compare theefficiencies of different somatic and zygotic explants starting from the tissue infection stage to plantregeneration and hardening.The results shows that compared to embryogeniccalli from anther and ovule a high transformation frequencyof 76.40%was obtained from embryonic calli derived from zygotic embryo. A clear advantage of zygotic explants wasapparent in different stages of plant regeneration and hardening also. Moreover, the embryos derived from this source had more number of lateralroots andshowed bettervigour than clonal explant. The results from our study indicate that, zygote derived target tissue are much better than somatic derived calli to overcome the problems associated with recalcitrance and lower regeneration frequencies often observed among transgenic perennial tree crops.

Paper Title:
Tumor inducing potential of agricultural soil samples of Amritsar (India)
Author Name:
Vanita Chahal, Upma Arora, Jatinder Kaur Katnoria
Author Email:
jkato8@yahoo.com
Country:
India
DOI:
https://doi.org/10.6084/m9.figshare.9783905.v1
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Tumor inducing potential of agricultural soil samples of Amritsar (India)
Author: Vanita Chahal, Upma Arora, Jatinder Kaur Katnoria

Soil is a three dimensional, dynamic natural body occurring on the surface of earth that provides all essential nutrients and mechanical anchorage to the growing plants. But, rapid industrialization, increasing population and inevitable use of xenobiotics have polluted the soil system. Contamination of soil is more prevalent in agricultural lands due to excessive use of agrochemicals like pesticides and inorganic fertilizers to obtain more crop yield. Upon entering the living systems via inhalation, ingestion and dermal contact, these chemicals cause a potential health risks. Considering the serious consequences of agricultural soil pollution on human health, the present study was planned to evaluate tumor inducing potential of agricultural soil samples collected from five different zones (Center, North, East, West and South) of Amritsar, Punjab (India) during cultivation of wheat and rice using potato disc tumor assay. It was found that SW-I soil sample induced maximum (24) tumors while Centre and North zone soil samplesinduced least number of tumors (3) during potato disc tumor assay. The present study clearly indicates the high risk to farmers as well as consumers if exposed to such polluted lands and consuming the contaminated crops.

Paper Title:
Recent advances in the fight against malaria
Author Name:
P.C. Mishra, Rachna Hora
Author Email:
pm.biotech@gndu.ac.in
Country:
India
DOI:
https://doi.org/10.6084/m9.figshare.9783908.v1
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Recent advances in the fight against malaria
Author: P.C. Mishra, Rachna Hora

Malaria is an infectious disease that dates as early as 2700 B.C., and is caused by a unicellular eukaryotic parasite ‘Plasmodium’. The word ‘malaria’ originates from the Italian phrase ‘mala aria’ meaning ‘bad air’. British physician Sir Ronald Ross identified genus Anopheles mosquitoes as vectors for transmission of this disease in 1897. There are more than 60 different species of Anopheles mosquitoes which are now known to be vectors of malaria parasite. Malaria is most widespread in the tropical countries with the African, South East Asian, Eastern Mediterranean and Western Pacific being defined as the endemic regions by World Health Organization (WHO). Spread of this disease in humans occurs through the bite of female anopheles mosquito infected with species Plasmodium falciparum (Pf), P. vivax(Pv), P. ovale(Po), P. malariae(Pm)or P. knowlesi(Pk). Of these, Pf causes the most lethal form of malaria which is responsible for most associated deaths especially in the Sub-Saharan African region. While Pf infections predominate the African continent, Pv and Pf-Pv mixed infections are more common in South-East Asia, including India. Globally, malaria accounts for nearly 0.4 million deaths annually with most victims being children less than 5 years of age. Common manifestations of the disease include high fever, shaking chills, anemia, respiratory distress etc. Though uncomplicated malaria is considered easily treatable with drugs, a large fraction of Pf infected patients develop severe complicated malaria. This may present itself as cerebral malaria causing seizures and coma. Neurological sequelaeresulting from cerebral malaria include cognitive defects, cortical blindness, deafness, epilepsy etc. leading to prolonged or permanent debilitation of the patients. Cerebral malaria is a form of malaria that results from cytoadherence of Plasmodium infected red blood cells on brain endothelium obstructing blood flow to the brain. Pregnant women residing in endemic areas are considered susceptible to placental malaria in which blood blow to the placental tissue is blocked by cytoadherence, leading to fetal mortality. While Pf is responsible for severe disease, Pv infection can cause recurrent malaria due to the formation of hypnozoites that lie dormant in the liver for long periods. These can be activated to show disease symptoms when favorable conditions occur.

Paper Title:
Isolation and Characterizations of Probiotics from Bovine (Cow) Milk
Author Name:
Atrayee Roy, Anindya S. Panja, Madhumita Maitra, Bidyut Bandopyadhyay
Author Email:
bidyut2006@gmail.com
Country:
India
DOI:
https://doi.org/10.6084/m9.figshare.9783911.v1
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Isolation and Characterizations of Probiotics from Bovine (Cow) Milk
Author: Atrayee Roy, Anindya S. Panja, Madhumita Maitra, Bidyut Bandopyadhyay

Products extracted from bovine (Cow) origin are the chief vehicle for the delivery of the probiotic. Probiotic is a potential therapeutic for numerous disease now-a-days. Our experiment was designed to find out the beneficial role of probiotic in cow milk. In this study, five isolates were selected from raw cow milk which was initially characterized through staining, different biochemical tests. All the isolates were shown Gram positive, non-endosporer rods in nature. CW1 and CW4 showed maximum bile salt tolerance including antimicrobial activity, whereas only CW1 showed maximum antioxidant activity. Furthermore molecular characterization (16S rRNA sequencing) and phylogenetic tree was analyzed. This unique microflora maybe used as a probiotic to enhance gastrointestinal health, prevention of pathogenic infection including immunomodulation.

Paper Title:
Molecular Approaches to the Diagnosis of Infectious Diseases
Author Name:
Sunik MaliK, Amarnath, Rajiv Khosla
Author Email:
khosla30@yahoo.com
Country:
India
DOI:
https://doi.org/10.6084/m9.figshare.9783914.v1
View PDF
Molecular Approaches to the Diagnosis of Infectious Diseases
Author: Sunik MaliK, Amarnath, Rajiv Khosla

Infectious diseases are the deadly processes being caused by a number of microbes. However, the timely detection of these agents can save many life’s. Although many biochemical techniques are available however, molecular approaches are far better to give authentic and timely results. The molecular diagnostic tools such as polymerase chain reaction (PCR) act as novel armamentarium in fight against existing as well as emerging infectious diseases. Moreover such an approach brings a new dimension in the early detection of infectious agents and would help in the better management thereby providing further strength to our pandemic preparedness. The intent of this review is to summarize the overview of molecular approaches to detect deadly infection agents.

Paper Title:
Vermitechnology: A tool to access and manage the toxicity of fly ash
Author Name:
Bhawana, Sharanpreet Singh, Jaswinder Singh, Adarsh Pal Vig
Author Email:
dr.adarshpalvig@gmail.com
Country:
India
DOI:
10.5281/zenodo.3459496
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Vermitechnology: A tool to access and manage the toxicity of fly ash
Author: Bhawana, Sharanpreet Singh, Jaswinder Singh, Adarsh Pal Vig

Fly ash(FA) is pulverized and burnt product of coal which is critical and toxic ceramic material for an environment. It contains various heavy metals which were harmful for soil health ultimately being a major reason for causing various ailments in humans.. The toxic effects of fly ash was seen on the different parameters of earthworms (Eisenia fetida) at higher concentrations. The physico-chemical properties and heavy metals analysis were done at the higher concentrations (50, 75 and100) of fly ash samples. It was found that pH and EC was very high at FA100. TOC was very high and TKN content was found very low at pure samples of fly ash (FA100). Heavy metals content was also high and almost cross the permissible limits at all concentrations. Thus, it was confirmed the localization of fly ash is hazardous to animals as well as the humans.

Paper Title:
Foldscope: An Efficient Tool for Quality Analysis of Seeds and Grains for Farmers
Author Name:
Arun dev Sharma, Priya Nischal
Author Email:
arundevsharma47@gmail.com
Country:
India
DOI:
10.5281/zenodo.3459500
View PDF
Foldscope: An Efficient Tool for Quality Analysis of Seeds and Grains for Farmers
Author: Arun dev Sharma, Priya Nischal

Foldscope is an ultra-affordable, paper microscope designed to be extremely portable, durable, and to give optical quality similar to conventional research microscopes. However its potential as in tool in the area of agricultural farmers is still not known. In this study, how foldscope can be used as an efficient tool for farmers in their fields was demonstrated. Seeds of wheat, maize, moong dal and black dal were purchased from local market. Imbibed seeds were half opened and stained in tetrazolium solution. Seed viability coupled with germination plays an fundamental role in plant growth, development and crop productivity. Therefore, foldscope images revealed viable red areas in the respective seeds, indicating live embryos. Based on data the use of foldscope was recommended.

Paper Title:
Foldscope based detection of thermophilic actinomycetes during composting process for Agaricus bisporus
Author Name:
Arun Dev Sharma, Inderjeet Kaur
Author Email:
arundevsharma47@gmail.com
Country:
India
DOI:
10.5281/zenodo.3524818
View PDF
Foldscope based detection of thermophilic actinomycetes during composting process for Agaricus bisporus
Author: Arun Dev Sharma, Inderjeet Kaur

Foldscope is the ultra-affordable, paper microscope designed to be extremely portable, durable, and to give optical quality similar to conventional research microscopes. However it’s potential as in tool in the area of agricultural sciences is still not known. In this study, how foldscope can be used as an efficient tool to detect actinomycetes in the compost preparation process of mushroom cultivation. Foldscope images revealed white colored actinomycetes. Based on data the use of foldscope was recommended.

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