Research & Reviews in Biotechnology and Biosciences

Archives Volume-1, Issue-1

Paper Title:: EFFECT OF PHOSPHORUS DEFICIENT CONDITIONS ON EXPRESSION OF ACID PHOSPHATASE (APASE) IN WHEAT TISSUES.
Author Name:: Parul Gulati, Arun Dev Sharma
Author Email:: arundevsharma47@gmail.com
Country:: India

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EFFECT OF PHOSPHORUS DEFICIENT CONDITIONS ON EXPRESSION OF ACID PHOSPHATASE (APASE) IN WHEAT TISSUES.

Author: Parul Gulati, Arun Dev Sharma

The effect of phosphorus stress on changes in acid phosphatase (APase) activities was studied in wheat seeds and shoots. Six-day old wheat seedlings were exposed to low phosphorus (LP) and high phosphorus (HP) stress by providing two different concentrations of 10μM and 1000μM KH2PO4 .The tissues (seeds and shoots) were separated . Treated and untreated extracts of seeds and shoots were compared. The Acid phosphatase activity was measured by using p-nitrophenyl phosphate (pNPP) as substrate, results revealed that APase activity was strongly induced under phosphorus stress. NATIVE PAGE APase activity also revealed a similar type of induction under phosphorus stress. SDS PAGE Analysis revealed that many polypeptides in treated extracts disappeared as compared to untreated extracts Based on these results; role of acid phosphatase (APase) was studied.

Paper Title:: MICROBIAL DECOLORIZATION OF TEXTILE DYE EFFLUENT
Author Name:: Purnima, Navleen Kaur Chopra, Dr. Jitender Kumar, Sumit Sharma and Sonia Sharma
Country:: India

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MICROBIAL DECOLORIZATION OF TEXTILE DYE EFFLUENT

Author: Purnima, Navleen Kaur Chopra, Dr. Jitender Kumar, Sumit Sharma and Sonia Sharma

Synthetic dyes are used in several industries including textile dyeing printing and cosmetic industries. Due to thier complex structure they are resistant to be decomposed by conventional treatment technologies. Hence microbial decolorization of dyes could be a viable option as low cost ecofriendly waste water treatment system from these industries. This study presents microbial decolorization of textile dye effluents from five microbial isolates isolated from five different effluent samples collected from fabric industry. Different parameters such as carbon source, temperature, pH and inoculum size were optimized for decolorization of Methylene Blue, Crystal Violet, EosinYellow, Safranine dyes by using bacterial isolates. Among carbon sources glucose was found to be the best decolorizer, 27°C-30°C of temperature was observed to be optimum for decolorization, the optimum pH range for decolorization was found to be from pH6-pH8 whereas 6% (v/v) of inoculum showed best decolorization results. All the samples were incubated at 30°C 100 rpm. The decolorization was measured as decrease in absorbance maxima at 663 nm,590 nm,518nm, 530 nm for mehtylene blue, crystal violet, eosin yellow, safranine respectively.

Paper Title:: PLANTS TO ENERGY: A REVIEW OF SOME POTENTIAL BIO-FUEL PLANTS OF JALANDHAR
Author Name:: Ruby Bhullar, Dr. Anjana Bhatia
Country:: India

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PLANTS TO ENERGY: A REVIEW OF SOME POTENTIAL BIO-FUEL PLANTS OF JALANDHAR

Author: Ruby Bhullar, Dr. Anjana Bhatia

In the era of increasing energy insecurity the first confront that we face is to move away from fossil fuels subsequently to build a new energy economy based on renewable source. Fossil fuels are not sustainable so the key issue for attaining sustainability is bio-based economy which can be achieved through biomass. Potential biomass feedstock includes conventional crops, forestry products, agricultural residue, waste material and specially cultivated energy-crops. An effort to identify higher plants from Jalandhar which may be useful as alternative source of fuel as energy-crop belonging to family Apocynaceae, Asclepideaceae, Asteraceae, Euphorbiaceae, Convolvulaceae, Fabaceae, Papaveraceae are analysed for their yield of petroleum-based products.

Paper Title:: POST HARVEST DETERIORATION OF SUGARCANE JUICE BY DEXTRANSUCRASE ISOLATED FROM LEUCONOSTOC MESENTEROIDES
Author Name:: Mani Sharma, APS Mann
Country:: India

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POST HARVEST DETERIORATION OF SUGARCANE JUICE BY DEXTRANSUCRASE ISOLATED FROM LEUCONOSTOC MESENTEROIDES

Author: Mani Sharma, APS Mann

The present studies were carried out to investigate the post harvest deterioration in sugarcane juice due to presence of Leuconostoc mesenteroides bacteria. The bacteria released sucrose hydrolyzing enzymes in the juice viz. invertase and dextransucrase, which hydrolyzed sucrose into glucose and fructose, thus reducing the sucrose content in the juice. Leuconostoc mesenteroides bacteria was isolated from slime obtained from two sugar mills at Budhewal (Broth I) and Morinda (Broth II). Purified strain no. 867 procured from institute of Microbial technology (IMTECH), Chandigarh was used as standard check. Sucrose hydrolyzing enzymes i.e. dextransucrase was isolated from L. mesenteroides. In addition to catalyzing the sucrose hydrolysis, dextransucrase also synthesized dextran due to its transferase activity. Maximum activity of the enzymes was associated with cell walls of the Leuconostoc mesenteroides isolated from Broth I, Broth II and standard 867. Effect of temperature was also studied on production of dextran by the bacteria islated from broth I and broth II.The present studies have potential applications in sugar industry for minimizing dextran problem and point to the need of estimating dextran levels and bacterial count, in sugarcane juice as a part of quality control for improving sugar recovery and sugar quality.

Paper Title:: IN VITRO DIFFERENTIATION FROM CULTURED EXPLANTS OF LEUCAENA LEUCOCEPHALA
Author Name:: V.K.Gautam, Nidhi Gautam
Author Email:: vkgautam2001@gmail.com
Country:: India

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IN VITRO DIFFERENTIATION FROM CULTURED EXPLANTS OF LEUCAENA LEUCOCEPHALA

Author: V.K.Gautam, Nidhi Gautam

The epicotyl and hypocotyl explants from in vitro grown seedlings were cultured on various media to exploit their morphogenetic potential using various plant growth regulators in various concentrations and combinations. In general leguminous trees are very much recalcitrant to morphogenetic studies. However, in this study both explants produced profuse calli as well as roots in most of the combinations tried. Very small shoots developed at very low frequency only in some combinations. Both explants produced a large amount of callus that can be used for regenerating plants in large numbers, i.e. indirect mode of organogenesis.
Key words: Hypocotyl, Epicotyl, in vitro, organogenesis

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